Cdc6 Antibody

Western Blot: Cdc6 Antibody [H00000990-D01P] - Analysis of CDC6 expression in transfected 293T cell line by CDC6 polyclonal antibody.Lane 1: CDC6 transfected lysate(62.70 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Cdc6 Antibody [H00000990-D01P] - Analysis of antibody to CDC6 on HeLa cell. Antibody concentration 10 ug/ml.

Proximity Ligation Assay: Cdc6 Antibody [H00000990-D01P] - Analysis of protein-protein interactions between CDC6 and CDKN1A. HeLa cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-CDKN1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: Cdc6 Antibody [H00000990-D01P] - Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: Cdc6 Antibody [H00000990-D01P] - Analysis of protein-protein interactions between CDC6 and PCNA. Mahlavu cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-PCNA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

Cdc6 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CDC6 (AAH25232.1, 1 a.a. - 560 a.a.) full-length human protein. MPQTRSQAQATISFPKRKLSRALNKAKNSSDAKLEPTNVQTVTCSPRVKALPLSPRKRLGDDNLCNTPHLPPCSPPKQGKKENGPPHSHTLKGRRLVFDNQLTIKSPSKRELAKVHQNKILSSVRKSQEITTNSEQRCPLKKESACVRLFKQEGTCYQQAKLVLNTAVPDRLPAREREMDVIRNFLREHICGKKAGSLYLSGAPGTGKTACLSRILQDLKKELKGFKTIMLNCMSLRTAQAVFPAIAQEICQEEVSRPAGKDMMRKLEKHMTAEKGPMIVLVLDEMDQLDSKGQDVLYTLFEWPWLSNSHLVLIGIANTLDLTDRILPRLQAREKCKPQLLNFPPYTRNQIVTILQDRLNQVSRDQVLDNAAVQFCARKVSAVSGDVRKALDVCRRAIEIVESDVKSQTILKPLSECKSPSEPLIPKRVGLIHISQVISEIDGNRMTLSQEGAQDSFPLQQKILVCSLMLLIRQLKIKEVTLGKLYEAYSKVCRKQQVAAVDQSECLSLSGLLEARGILGLKRNKETRLTKVFFKIEEKEIEHALKDKALIGNILATGLP
• Specificity: CDC6 - cell division cycle 6 homolog (S. cerevisiae),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cdc6 Antibody

• CDC18LHsCDC18
• Cdc18-related protein
• CDC6 (cell division cycle 6, S. cerevisiae) homolog
• CDC6 cell division cycle 6 homolog (S. cerevisiae)
• CDC6 cell division cycle 6 homolog
• CDC6-related protein
• cell division control protein 6 homolog
• cell division cycle 6 homolog (S. cerevisiae)
• cell division cycle 6 protein
• HsCdc18
• HsCDC6
• p62(cdc6)

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC6
• Entrez:
  • 990(Human)
• Uniprot:
  • AAH25232.1(Human)