CDC45L Antibody (JJ091-04)

Immunocytochemistry/Immunofluorescence: CDC45L Antibody (JJ091-04) [NBP2-67897] - Staining CDC45 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDC45L Antibody (JJ091-04) [NBP2-67897] - Analysis of paraffin-embedded mouse kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.

Flow Cytometry: CDC45L Antibody (JJ091-04) [NBP2-67897] - Analysis of 293 cells with CDC45 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CDC45L Antibody (JJ091-04) [NBP2-67897] - Staining CDC45 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC45L Antibody (JJ091-04) [NBP2-67897] - Staining CDC45 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDC45L Antibody (JJ091-04) [NBP2-67897] - Analysis of paraffin-embedded human kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CDC45L Antibody (JJ091-04) [NBP2-67897] - Analysis of paraffin-embedded mouse colon tissue using anti-CDC45 antibody. Counter stained with hematoxylin.

Product Discontinued

CDC45L Antibody (JJ091-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic protein within Human CDC45L aa 501-544 / 566. (SwissProt: O75419 Human; SwissProt: Q9Z1X9 Mouse; Entrez Gene: 287961 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC45
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC45L Antibody (JJ091-04)

• CDC45 cell division cycle 45-like (S. cerevisiae)
• cell division cycle 45 homolog (S. cerevisiae)
• cell division cycle 45-like 2
• human CDC45
• S.cerevisiae, homolog)-like

Background

CDC45L is encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDC45L Antibody (NBP2-67897)(1)

Publication using NBP2-67897

• Kochenova OV, Mukkavalli S, Raman M, Walter JC Cooperative assembly of p97 complexes involved in replication termination Nature communications 2022-11-03 [PMID: 36329031] (WB)
  
  Details:
  Dilution used in WB 1:1000

Bioinformatics

• Gene Symbol: CDC45