Cdc27 Antibody (SD85-02)

Western Blot: Cdc27 Antibody (SD85-02) [NBP2-67828] - Analysis of Cdc27 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate Predicted band size: 92 kDa Observed band size: 85 kDa

Immunohistochemistry-Paraffin: Cdc27 Antibody (SD85-02) [NBP2-67828] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cdc27 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Cdc27 Antibody (SD85-02) [NBP2-67828] - Analysis of Cdc27 on different lysates using anti-Cdc27 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562

Immunohistochemistry-Paraffin: Cdc27 Antibody (SD85-02) [NBP2-67828] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cdc27 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Cdc27 Antibody (SD85-02) [NBP2-67828] - Analysis of paraffin-embedded human prostate tissue with Rabbit anti-Cdc27 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Cdc27 Antibody (SD85-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Cdc27 aa 662-824 / 824. (SwissProt: P30260 Human; SwissProt: A2A6Q5 Mouse; SwissProt: Q4V8A2 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC27
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cdc27 Antibody (SD85-02)

• ANAPC3CDC27 homolog
• anaphase promoting complex subunit 3
• Anaphase-promoting complex subunit 3
• anaphase-promoting complex, protein 3
• APC 3
• APC3cell division cycle 27
• CDC27Hs
• cell division cycle 27 homolog (S. cerevisiae)
• cell division cycle protein 27 homolog
• D0S1430E
• D17S978E
• HNUC
• H-NUC
• nuc2 homolog
• NUC2

Background

Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC27