Cdc27 Antibody (AF 3.1) [Allophycocyanin/Cy7]

Product Discontinued

Cdc27 Antibody (AF 3.1) [Allophycocyanin/Cy7] Summary

• Immunogen: A peptide corresponding to the C-terminal 10 residues of human cdc27 coupled to KLH.
• Localization: Nuclear
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC27
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for Cdc27 Antibody (AF 3.1) [Allophycocyanin/Cy7]

• ANAPC3CDC27 homolog
• anaphase promoting complex subunit 3
• Anaphase-promoting complex subunit 3
• anaphase-promoting complex, protein 3
• APC 3
• APC3cell division cycle 27
• CDC27Hs
• cell division cycle 27 homolog (S. cerevisiae)
• cell division cycle protein 27 homolog
• D0S1430E
• D17S978E
• HNUC
• H-NUC
• nuc2 homolog
• NUC2

Background

Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC27