Cdc23 Antibody (1Q2I3)

Western Blot: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Western blot analysis of extracts of various cell lines, using Cdc23 Rabbit mAb (NBP3-16631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Immunofluorescence analysis of HeLa cells using Cdc23 Rabbit mAb (NBP3-16631) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Immunofluorescence analysis of HeLa cells using Cdc23 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 1Q2I3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cdc23 Antibody (1Q2I3) Summary

• Description: Novus Biologicals Rabbit Cdc23 Antibody (1Q2I3) (NBP3-16631) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 498-597 of human CDC23 (Q9UJX2). YSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC23
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Cdc23 Antibody (1Q2I3)

• ANAPC8anaphase promoting complex subunit 8
• Anaphase-promoting complex subunit 8
• APC8CDC23 (cell division cycle 23, yeast, homolog)
• cell division cycle 23 homolog (S. cerevisiae)
• cell division cycle protein 23 homolog
• CUT23
• Cyclosome subunit 8

Background

CDC23/APC8 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC23