Cdc20 Antibody (BA8) - BSA Free

• Reactivity: Xp
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: BA8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Cdc20 Antibody (BA8) - BSA Free Summary

• Immunogen: Peptide from the carboxy terminus of the protein.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC20
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Western Blot 1:100 - 1:2000
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cdc20 Antibody (BA8) - BSA Free

• CDC20 cell division cycle 20 homolog (S. cerevisiae)
• CDC20 cell division cycle 20 homolog
• Cdc20
• cell division cycle 20 homolog (S. cerevisiae)
• cell division cycle protein 20 homolog
• MGC102824
• p55CDC
• p55CDCS. cerevisiae, homolog)

Background

CDC20 is an activating regulatory factor for the APC/C (anaphase promoting complex/cyclosome). It activates the ubiquitination activity of the APC/C. CDC20 confers a strict destruction-box (D-box) dependence on APC. Levels of CDC20, as well as its binding to APC, peak in mitosis and decrease drastically at early G1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cdc20 Antibody (NB100-2646)(2)

Publications using NB100-2646

• Bancroft J, Holder J, Geraghty Z et al. PP1 promotes cyclin B destruction and the metaphase-anaphase transition by dephosphorylating CDC20 Molecular Biology of the Cell 2020-10-01 [PMID: 32755477] (Western Blot, Frog - Xenopus (African Clawed Frog))
• Sackton KL, Dimova N, Zeng X et al. Synergistic blockade of mitotic exit by two chemical inhibitors of the APC/C. Nature. 2014-08-24 [PMID: 25156254] (WB, Xenopus)

Bioinformatics

• Gene Symbol: CDC20
• Entrez:
  • 991(Human)