EMR2 Antibody

Recombinant Human EMR2 (Catalog # 4894-EM), immobilized onto a microplate, supports the adhesion of the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human EMR2 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894). The ND50 is typically 1.5-6 µg/mL.

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMR2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

EMR2 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human EMR2
  Gln24-Gln478 (Gln24 predicted)
  Accession # NP_038475
• Specificity: Detects human EMR2 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 1% cross-reactivity with recombinant mouse EMR1.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: EMR2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Neutralization
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMR2 Antibody

• CD312
• DKFZp781B135
• egf-like module containing, mucin-like, hormone receptor-like 2
• egf-like module containing, mucin-like, hormone receptor-like sequence 2
• EGF-like module receptor 2
• EGF-like module-containing mucin-like hormone receptor-like 2
• EMR2

Background

EMR2 (EGF-like module-containing mucin-like receptor 2; designated CD312) is a glycoprotein belonging to the EGF-TM7 family of adhesion-type class B 7‑transmembrane (TM) receptors. EGF-like sequences within long extracellular N-termini, and a GPS (G-protein proteolytic site) domain are characteristic of this family, which is mainly expressed on cells of the immune system (1, 2). The human EMR2 cDNA encodes an 823 amino acid (aa) protein with five EGF-like domains within the first 250 aa, followed by a mucin-like stalk, a GPS domain (aa 479-530) and a 7-TM sequence (aa 531-785). The GPS domain is the site of autocatalytic cleavage, forming two cleaved portions that remain non-covalently attached as a heterodimer (1, 3). Of the first 290 aa of human EMR2, 284 aa (97%) are identical with family member CD97, likely due to gene duplication (2). The portion of human EMR2 N-terminal to the GPS domain (aa 1-478) shares 64%, 59%, 48% and 45% aa identity with corresponding regions of canine EMR2, equine EMR2, mouse CD97 and rat CD97, respectively. Alternate splicing of EMR2 creates isoforms that contain 2-5 EGF-like domains. Only the 5-EGF form contains EGF4, which is necessary for calcium-dependent binding of the EMR2/CD97 ligand, chondroitin sulfate (CS) (2, 4‑6). None of the isoforms engage the CD97 ligand, CD55 (DAF). EMR2 is restricted to myeloid cells (1, 2). EMR2 expression increases as monocytes differentiate into macrophages, and decreases with differentiation into dendritic cells (5). Activation increases neutrophil EMR2 expression (5). EMR2 localizes to the leading edge of migrating neutrophils and plays an important role in migration, adhesion and superoxide production (7). It is also thought to facilitate specific interaction of myeloid cells with peripheral B lymphocytes which express CS (6).

1. Kwakkenbos, M.J. et al. (2004) Immunogenetics 55:655.
2. Lin, H.-H. et al. (2000) Genomics 67:188.
3. Lin, H.-H. et al. (2004) J. Biol. Chem. 279:31823.
4. Stacey, M. et al. (2003) Blood 102:2916.
5. Chang G.-W. et al. (2007) Biochem. Biophys. Res. Commun. 353:133.
6. Kwakkenbos, M.J. et al. (2005) J. Leukoc. Biol. 77:112.
7. Yona, S. et al. (2008) FASEB J. 22:741.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EMR2 Antibody (AF4894)(2)

Publications using AF4894

• S Irmscher, SLH Zipfel, LD Halder, L Ivanov, A Gonzalez-D, C Waldeyer, M Seiffert, FJ Brunner, M von der He, I Löschmann, S Wulf, D Czamara, N Papac-Mili, O Strau beta , S Lorkowski, H Reichenspu, MV Holers, NK Banda, T Zeller, EB Binder, CJ Binder, T Wiech, PF Zipfel, C Skerka Factor H-related protein 1 (FHR-1) is associated with atherosclerotic cardiovascular disease Scientific Reports, 2021;11(1):22511. 2021 [PMID: 34795372] (Neutralization, Human)
• N Bhudia, S Desai, N King, N Ancellin, D Grillot, AA Barnes, SJ Dowell G Protein-Coupling of Adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and Activation of G Protein Signalling by an Anti-EMR2 Antibody Sci Rep, 2020;10(1):1004. 2020 [PMID: 31969668] (Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: EMR2
• Uniprot:
  • NP_038475()