CD96 Antibody (628211) [Unconjugated] - v2

Human peripheral blood cells were stained with Human CD96 Monoclonal Antibody (Catalog # MAB6199) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B) and Human CD3 epsilon Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 628211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD96 Antibody (628211) [Unconjugated] - v2 Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD96
  Val22-Asp501
  Accession # NP_005807
• Specificity: Detects human CD96 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD96
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD96 Antibody (628211) [Unconjugated] - v2

• CD96 antigenMGC22596
• CD96 molecule
• CD96
• DKFZp667E2122
• T cell-activated increased late expression protein
• TACTILE
• TACTILEincreased late expression
• T-cell surface protein tactile

Background

CD96 (also known as Tactile) is a 160 kDa type I transmembrane glycoprotein that contains three Ig-like domains in its extracellular region. It is expressed on CD4⁺ and CD8⁺ T cells, NK cells, and select B cells. CD96 binds to CD155/PVR and participates in NK cell-mediated lysis of CD155⁺ target cells. Alternate splicing generates a short variant (CD96v2) which lacks 16 amino acids (aa) within the second Ig-like domain. CD96v2 is the predominant isoform in many cell types and exhibits even greater binding affinity with CD155 than does full length CD96. A soluble form of CD96 circulates in the serum. Over aa 22-501, human CD96v2 shares 55% aa sequence identity with mouse and rat CD96.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD96 Antibody (MAB6199)(1)

Publication using MAB6199

• A Rogel, FM Ibrahim, SM Thirdborou, F Renart-Dep, CN Birts, SL Buchan, X Preville, EV King, A Al-Shamkha Fc-gamma receptor-mediated crosslinking co-defines the immunostimulatory activity of anti-human CD96 antibodies JCI Insight, 2022-10-10;0(0):. 2022-10-10 [PMID: 35998045] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD96
• Uniprot:
  • NP_005807()