CD84/SLAMF5 Antibody (CD84.1.21) [FITC]

Flow Cytometry: CD84/SLAMF5 Antibody (CD84.1.21) [FITC] - [NB100-64062] - Figure A. Alexa Fluor 700 conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD84. All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock. Data acquired on the ZE5 Cell Analyzer.

• Reactivity: Hu
• Applications: Flow
• Clone: CD84.1.21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC
• Concentration: 0.1 mg/ml

CD84/SLAMF5 Antibody (CD84.1.21) [FITC] Summary

• Immunogen: Mouse pre-B cell line 300.19 transfected with CD84 cDNA
• Localization: Type I membrane protein
• Specificity: NB100-65929 recognizes the human CD84 cell surface antigen, a highly glycosylated cell surface antigen of 64-82kD. CD84 is predominantly expressed by B lymphocytes, monocytes and macrophages, although lower levels of expression have also been reported on T cells, granulocytes and platelets.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD84
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Control:

  CD84/SLAMF5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% Bovine Serum Albumin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for CD84/SLAMF5 Antibody (CD84.1.21) [FITC]

• CD84 antigen (leukocyte antigen)
• CD84 antigen
• CD84 molecule
• CD84
• Cell surface antigen MAX.3
• DKFZp781E2378
• hCD84
• hly9-beta
• leucocyte differentiation antigen CD84
• leukocyte antigen CD84
• Leukocyte differentiation antigen CD84
• Ly-9B
• mCD84
• Signaling lymphocytic activation molecule 5
• SLAM family member 5
• SLAMF5
• SLAMF5LY9B

Background

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production. CD84 is expressed on mature B cells and B cell lines but not on plasma cell lines. Immunohistochemical studies demonstrate that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T cells. Cellular expression does not significantly increase after activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD84
• Entrez:
  • 8832(Human)
• Uniprot:
  • Q9UIB8(Human)