CD74 Antibody (OTI1H3) - Azide and BSA Free

Images

 
Western Blot: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74.
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI1H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

CD74 Antibody (OTI1H3) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CD74(NP_001020330) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD74
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:4000
  • Immunohistochemistry 1:150
Theoretical MW
33.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for CD74 Antibody (OTI1H3) - Azide and BSA Free

  • CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
  • CD74 antigen
  • CD74 molecule, major histocompatibility complex, class II invariant chain
  • CD74
  • CLIP
  • DHLAG
  • DHLAGgamma chain of class II antigens
  • HLA class II histocompatibility antigen gamma chain
  • HLADG
  • HLA-DR antigens-associated invariant chain
  • HLA-DR-gamma
  • Ia antigen-associated invariant chain
  • Ia-associated invariant chain
  • Ia-gamma
  • Ii
  • INVG34
  • MHC HLA-DR gamma chain
  • p33

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD74 Antibody (NBP2-70382) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70382AF350
Alexa Fluor 405 NBP2-70382AF405
Alexa Fluor 488 NBP2-70382AF488
Alexa Fluor 532 NBP2-70382AF532
Alexa Fluor 594 NBP2-70382AF594
Alexa Fluor 647 NBP2-70382AF647
Alexa Fluor 700 NBP2-70382AF700
Alexa Fluor 750 NBP2-70382AF750
Biotin NBP2-70382B
DyLight 350 NBP2-70382UV
DyLight 405 NBP2-70382V
DyLight 488 NBP2-70382G
DyLight 550 NBP2-70382R
DyLight 594 NBP2-70382DL594
DyLight 650 NBP2-70382C
DyLight 680 NBP2-70382FR
DyLight 755 NBP2-70382IR
FITC NBP2-70382F
HRP NBP2-70382H
Janelia Fluor 549 NBP2-70382JF549
Janelia Fluor 646 NBP2-70382JF646

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Blogs on CD74.

CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.

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Bioinformatics

Gene Symbol CD74