CD74 Antibody (6D9) - BSA Free

Western Blot: CD74 Antibody (6D9) [NBP2-59698] - Analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.

Immunocytochemistry/Immunofluorescence: CD74 Antibody (6D9) [NBP2-59698] - Analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 6D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD74 Antibody (6D9) - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms
• Localization: Cell Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane , Golgi Apparatus , Endosome , Lysosome
• Specificity: Detects approx 50kDa. No cross-reactivity against rat Cab Beta1b, Cav beta2a, and Cav betas3 in transfected cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD74
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: A 1:1000 dilution of this antibody was sufficient for detection of CD74 in 15 ug of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD74 Antibody (6D9) - BSA Free

• CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
• CD74 antigen
• CD74 molecule, major histocompatibility complex, class II invariant chain
• CD74
• CLIP
• DHLAG
• DHLAGgamma chain of class II antigens
• HLA class II histocompatibility antigen gamma chain
• HLADG
• HLA-DR antigens-associated invariant chain
• HLA-DR-gamma
• Ia antigen-associated invariant chain
• Ia-associated invariant chain
• Ia-gamma
• Ii
• INVG34
• MHC HLA-DR gamma chain
• p33

Background

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment.Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD74