CD57 Antibody (TB01) [Alexa Fluor® 647]

Images

 
Product Image: CD57 Antibody (TB01) [Alexa Fluor® 647] [NBP2-62203AF647] - Vial of Alexa Fluor 647 conjugated antibody. Alexa Fluor 647 is optimally excited at 653 nm by the Red laser (633 or 640 nm) and has an ...read more

Product Details

Summary
Product Discontinued
View other related CD57 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-62203AF647
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

CD57 Antibody (TB01) [Alexa Fluor® 647] Summary

Immunogen
A pool of neuroblastoma cell lines
Specificity
This antibody recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
B3GAT1
Purity
Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Chromatography

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD57 Antibody (TB01) [Alexa Fluor® 647]

  • CD57
  • HNK1
  • HNK-1
  • Human Natural Killer-1
  • L2
  • LEU7
  • LEU-7

Background

The CD57 antigen, also referred to as human natural killer-1 (HNK-1) and LEU-7, is a carbohydrate epitope containing sulfated glucuronic acid that is expressed by many cell types within the nervous system (1,2). Cellular expression of CD57 changes during progression of neural development (1). The CD57 epitope is synthesized by enzymes including a sulfotransferase and one of two glucotransferases (GlcAT-P or GlcAT-S) (1,2). The structure of the CD57/HNK-1 epitope consists of 3-O-sulfated glucuronic acid (GlcA) attached to a terminal galactose (HSO3-3GlcAbeta1-3Galbeta1-4GlcNAc-R) (1,2). The CD57 epitope was first identified and a marker for HNK cells and its expression is associated with NK cell maturation (1-3).

CD57 expression has become a common pathological marker. CD57+ CD8+ T cell expression is observed in various cancers and is often linked to reduced survival (3). On the other hand, CD57+ NK cell expression is associated with less severe disease and better prognosis in cancer patients (3). CD57 is also associated with immune dysfunction and autoimmune disease (3,4). For example, T cell immunosenescence is characterized by an upregulation of CD57 along with KLRG-1 and a loss of CD27 and CD28 expression (4). Expanded autoreactive T cells are also related to diseases including multiple sclerosis (MS) and rheumatoid arthritis (RA) (3). Conversely, reduced numbers of CD57+ NK cells and NK cell cytotoxicity is observed in various autoimmune diseases such as apoptotic dermatitis and sjogren's syndrome (3).

References

1. Focosi D, Bestagno M, Burrone O, Petrini M. CD57+ T lymphocytes and functional immune deficiency. J Leukoc Biol. 2010;87(1):107-116. https://doi.org/10.1189/jlb.0809566

2. Morise J, Takematsu H, Oka S. The role of human natural killer-1 (HNK-1) carbohydrate in neuronal plasticity and disease. Biochim Biophys Acta Gen Subj. 2017;1861(10):2455-2461. https://doi.org/10.1016/j.bbagen.2017.06.025

3. Nielsen CM, White MJ, Goodier MR, Riley EM. Functional Significance of CD57 Expression on Human NK Cells and Relevance to Disease. Front Immunol. 2013;4:422. Published 2013 Dec 9. https://doi.org/10.3389/fimmu.2013.00422

4. Lian J, Yue Y, Yu W, Zhang Y. Immunosenescence: a key player in cancer development. J Hematol Oncol. 2020;13(1):151. Published 2020 Nov 10. https://doi.org/10.1186/s13045-020-00986-z6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB4375
Species: Hu, Mu, Rt
Applications: IHC
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr,  IHC-P, IP, InhibTFunc
7268-CT
Species: Hu
Applications: BA
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC,  IHC-P, PA, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr,  IHC-P, IP, WB
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr,  IHC-P, Simple Western, WB
202-IL
Species: Hu
Applications: BA
MAB4077
Species: Hu
Applications: IHC, WB
AF2408
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, KO, Simple Western, WB
NB600-1441
Species: Ca, Hu, Mu, Po
Applications: Flow, ICC/IF, IHC, IHC-Fr,  IHC-P, KD
6507-IL/CF
Species: Hu
Applications: BA
AF1597
Species: Hu
Applications: Block, WB
4325-FC
Species: Hu
Applications: Bind
MAB342
Species: Hu
Applications: AgAct, ICC, WB
AF114
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
NB300-201
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr,  IHC-P, WB

Publications for CD57 Antibody (NBP2-62203AF647) (0)

There are no publications for CD57 Antibody (NBP2-62203AF647).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD57 Antibody (NBP2-62203AF647) (0)

There are no reviews for CD57 Antibody (NBP2-62203AF647). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD57 Antibody (NBP2-62203AF647) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CD57 Products

Array NBP2-62203AF647

Research Areas for CD57 Antibody (NBP2-62203AF647)

Find related products by research area.

Blogs on CD57

There are no specific blogs for CD57, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CD57 Antibody (TB01) [Alexa Fluor® 647] and receive a gift card or discount.

Bioinformatics

Gene Symbol B3GAT1