CD5 Antibody (UCHT2)

Flow Cytometry: CD5 Antibody (UCHT2) [NBP1-43452] - Analysis using the PE/Cy7 conjugate of NBP1-43452. Staining of normal human peripheral blood cells with Anti-Human CD19 APC and Mouse IgG1 k Isotype Control PE-Cy7 (cat. 25-4714) (left) or Anti-Human CD5 PE-Cy7 (right).

Flow Cytometry: CD5 Antibody (UCHT2) [NBP1-43452] - Staining of normal human peripheral blood cells with Anti-Human CD5 FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Flow Cytometry: CD5 Antibody (UCHT2) [NBP1-43452] - Analysis using the PE conjugate of NBP1-43452. Analysis of human peripheral blood leukocytes using CD5 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.

Product Discontinued

CD5 Antibody (UCHT2) Summary

• Immunogen: The immunogen for this antibody was CD5.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: The UCHT2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Human, and recognizes Primate CD 5.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD5 Antibody (UCHT2)

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation. The monoclonal antibody UCHT2 recognizes primate CD5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD5