CD5 Antibody (UCHT2) [PE/Cy7]

Flow Cytometry: CD5 Antibody (UCHT2) [PE/Cy7] [NBP1-42972] - Staining of normal human peripheral blood cells with Anti-Human CD19 APC and Mouse IgG1 k Isotype Control PE-Cy7 (cat. 25-4714) (left) or Anti-Human CD5 PE-Cy7 (right). Cells in the lymphocyte gate were used for analysis.

Product Discontinued

CD5 Antibody (UCHT2) [PE/Cy7] Summary

• Immunogen: The immunogen for this antibody was CD5.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: The UCHT2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Human, and recognizes Primate CD 5.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD5 Antibody (UCHT2) [PE/Cy7]

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD5