CD5 Antibody (2-4-2B1)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
2-4-2B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

CD5 Antibody (2-4-2B1) Summary

Immunogen
The antibody reacts with the CD5-antigen antigen.
Specificity
This monoclonal antibody recognizes rat CD5 antigen (MW 69 kDa) and is reactive with all common inbred strains. 2-4-2B1 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD5
Purity
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
This antibody can be used for flow cytometry and immunohistochemistry on frozen sections.For flow cytometry and immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10 for flow cytometry

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.01% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD5 Antibody (2-4-2B1)

  • CD5 antigen (p56-62)
  • CD5 antigen
  • CD5 molecule
  • CD5
  • LEU1T-cell surface glycoprotein CD5
  • Lymphocyte antigen T1/Leu-1
  • T1

Background

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD5
Entrez