CD46 Antibody (MM10)

• Reactivity: Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: MM10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

CD46 Antibody (MM10) Summary

• Immunogen: Mouse CD46-human Fc fusion protein
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD46
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Western Blot
• Application Notes: For Immunohistochemistry, Flow Cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilution. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for CD46 Antibody (MM10)

• AHUS2
• CD46 antigen
• CD46 molecule, complement regulatory protein
• CD46
• MCP
• membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
• MIC10
• TLXcomplement regulatory protein
• trophoblast leucocyte common antigen
• Trophoblast leukocyte common antigen
• trophoblast-lymphocyte cross-reactive antigen

Background

The monoclonal antibody MM10 recognizes membrane cofactor protein (MCP), also known as CD46. MCP is a type I membrane glycoprotein that consists of four isoforms i.e. two C-isoforms (51-58 kDa) and two BC isoforms (59-68 kDa) which arise by alternative splicing of a single CD46 gene. Membrane cofactor protein is expressed on every cell and tissue, with the exception of erythrocytes. Membrane cofactor protein serves to down-regulate the activation of complement on host tissue. MCP is a cofactor for factor 1-mediated cleavage of C3b and C4b and this way serves to protect the host cell against autologous attack. MCP is a receptor for several pathogens including measles virus, group A Streptococcus pyogenes and pathogenic Neisseria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD46