CD39L4/ENTPD5 Antibody (064) - Azide and BSA Free

Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa

Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human liver with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human kidney with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - ENTPD5 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL NBP2-89830 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP2-89830 at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 47 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: 064
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CD39L4/ENTPD5 Antibody (064) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD39L4/ENTPD5 (Uniprot#: O75356; Met1-His428).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ENTPD5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000
• Application Notes: This antibody will detect Human CD39L4/ENTPD5 in ELISA pair set (Catalog: # NBP2-79350). In a sandwich ELISA, it can be used as a capture antibody when paired with (Catalog: # NBP2-89829).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CD39L4/ENTPD5 Antibody (064) - Azide and BSA Free

• CD39 antigen-like 4
• CD39L4
• CD39L4NTPDase 5
• CD39-like 4
• EC 3.6.1
• EC 3.6.1.42
• EC 3.6.1.6
• ectonucleoside triphosphate diphosphohydrolase 5
• ENTPD5
• ER-UDPase
• GDPase ENTPD5
• Guanosine-diphosphatase ENTPD5
• MGC163357
• MGC163359
• NTPDase 5
• NTPDase-5
• Nucleoside diphosphatase
• Pcph proto-oncogene protein
• PCPH
• proto-oncogene CPH
• UDPase ENTPD5
• Uridine-diphosphatase ENTPD5

Background

ENTPD5 is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENTPD5