CD39L1/ENTPD2 Antibody [Unconjugated]

Western blot shows lysates of mouse salivary gland tissue and rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5797) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD39L1/ENTPD2 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L1/ENTPD2
  Cys26-Ser462
  Accession # O55026
• Specificity: Detects mouse and rat CD39L1/ENTPD2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) CD39, rmCD39L2, and rmCD39L3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ENTPD2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39L1/ENTPD2 Antibody [Unconjugated]

• CD39 antigen-like 1
• CD39L1
• CD39L1NTPDase 2
• CD39-like-1
• EC 3.6.1
• EC 3.6.1.-
• EC 3.6.1.5
• Ecto-ATP diphosphohydrolase 2
• Ecto-ATPase 2
• Ecto-ATPase
• Ecto-ATPDase 2
• ectonucleoside triphosphate diphosphohydrolase 2
• ENTPD2
• NTPD2
• NTPDase 2
• NTPDase-2

Background

CD39L1, also known as ENTPD2 and NTPDase2, is an ecto-nucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells (1). CD39L1 is a Ca²⁺- and Mg²⁺-dependent enzyme that activates platelets by preferentially converting ATP to ADP (2). CD39L1 plays a role in regulating thrombosis and inflammation (3). It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation (2, 4).

1. Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on Ecto-ATPases and Related Ectonucleotidases:18.        
2. Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.  
3. Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.  
4. Sevigny, J. et al. 2002 Blood 99:2801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD39L1/ENTPD2 Antibody (AF5797)(3)

Publications using AF5797

• N Shigemura, S Takai, F Hirose, R Yoshida, K Sanematsu, Y Ninomiya Expression of Renin-Angiotensin System Components in the Taste Organ of Mice Nutrients, 2019;11(9):. 2019 [PMID: 31546789] (IHC-Fr, Mouse)
• W Yu, WG Hill, SC Robson, ML Zeidel Role of P2XReceptor in Mouse Voiding Function Sci Rep, 2018;8(1):1838. 2018 [PMID: 29382907] (IHC-Fr, Mouse)
• Yu W, Robson SC, Hill WG Expression and distribution of ectonucleotidases in mouse urinary bladder. PLoS ONE, 2011;6(4):e18704. 2011 [PMID: 21533188] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ENTPD2
• Uniprot:
  • O55026()