CD39L1/ENTPD2 Antibody [Unconjugated]

Western blot shows lysates of mouse salivary gland tissue and rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5797) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

NTPDase2 immunostaining co-localize with vimentin, desmin, and PDGF beta receptor in mice bladder.Cryosections of mouse bladders were labeled with antibodies to vimentin (A. green), desmin (D. green), PDGF beta receptor (G. green), and NTPDase2 (B. E. H. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C. F. I). Merged signals of NTPDase2 and vimentin, desmin, and PDGF beta receptor are shown as yellow (C. F. I). White arrows indicate representative co-localization. White asterisks indicate non-co-localized signal of smooth muscle (I) and fibroblasts (F. I). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

NTPDase2 co-localizes with CD34 but not tryptase in mouse bladder.Cryosections of mouse bladders were labeled with antibodies to CD34 (A, D. green), NTPDase2 (B. red), tryptase (E. red), c-kit (G. green), and Topro-3 to label nuclei (B. E. H. blue). Color merged panels are shown on the right (C, F, I). Merged signals are shown as yellow (C. F. I). White arrows indicate representative NTPDase2/CD34 co-localization (C) or CD34/tryptase co-localization (F); White asterisks indicate CD34 positive cells with no NTPDase2 staining (C). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

NTPDase2 immunostaining co-localize with vimentin, desmin, and PDGF beta receptor in mice bladder.Cryosections of mouse bladders were labeled with antibodies to vimentin (A. green), desmin (D. green), PDGF beta receptor (G. green), and NTPDase2 (B. E. H. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C. F. I). Merged signals of NTPDase2 and vimentin, desmin, and PDGF beta receptor are shown as yellow (C. F. I). White arrows indicate representative co-localization. White asterisks indicate non-co-localized signal of smooth muscle (I) and fibroblasts (F. I). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

NTPDase2 immunostaining does not co-localize with alpha -SMA in mice bladder smooth muscle.Cryosections of mouse bladders were labeled with antibodies to NTPDase2 (A. green), alpha -SMA (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). White arrows indicate distinct NTPDase2 staining next to bladder smooth muscle cells. White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

NTPDase2 immunostaining co-localize with c-kit in mice bladder.Cryosections of mouse bladders were labeled with antibodies to NTPDase2 (B. red), c-kit (A. green) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and c-kit are shown as yellow (C). White arrows indicate representative NTPDase2/c-kit co-localization. White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

NTPDase2 immunostaining co-localize with connexin 43 in mice bladder.Cryosections of mouse bladders were labeled with antibodies to connexin 43 (A. green), NTPDase2 (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and connexin 43 are shown as yellow (C). White arrows indicate representative NTPDase2/connexin 43 co-localization. White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

NTPDase2 immunostaining co-localize with vimentin, desmin, and PDGF beta receptor in mice bladder.Cryosections of mouse bladders were labeled with antibodies to vimentin (A. green), desmin (D. green), PDGF beta receptor (G. green), and NTPDase2 (B. E. H. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C. F. I). Merged signals of NTPDase2 and vimentin, desmin, and PDGF beta receptor are shown as yellow (C. F. I). White arrows indicate representative co-localization. White asterisks indicate non-co-localized signal of smooth muscle (I) and fibroblasts (F. I). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

NTPDase2 immunostaining co-localize with merlin/NF2 in mice bladder.Cryosections of mouse bladders were labeled with antibodies to merlin/NF2 (A. green), NTPDase2 (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and merlin/NF2 are shown as yellow (C). White arrows indicate representative NTPDase2/merlin co-localization. White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD39L1/ENTPD2 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L1/ENTPD2
  Cys26-Ser462
  Accession # O55026
• Specificity: Detects mouse and rat CD39L1/ENTPD2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) CD39, rmCD39L2, and rmCD39L3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ENTPD2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39L1/ENTPD2 Antibody [Unconjugated]

• CD39 antigen-like 1
• CD39L1
• CD39L1NTPDase 2
• CD39-like-1
• EC 3.6.1
• EC 3.6.1.-
• EC 3.6.1.5
• Ecto-ATP diphosphohydrolase 2
• Ecto-ATPase 2
• Ecto-ATPase
• Ecto-ATPDase 2
• ectonucleoside triphosphate diphosphohydrolase 2
• ENTPD2
• NTPD2
• NTPDase 2
• NTPDase-2

Background

CD39L1, also known as ENTPD2 and NTPDase2, is an ecto-nucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells (1). CD39L1 is a Ca²⁺- and Mg²⁺-dependent enzyme that activates platelets by preferentially converting ATP to ADP (2). CD39L1 plays a role in regulating thrombosis and inflammation (3). It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation (2, 4).

1. Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on Ecto-ATPases and Related Ectonucleotidases:18.        
2. Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.  
3. Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.  
4. Sevigny, J. et al. 2002 Blood 99:2801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD39L1/ENTPD2 Antibody (AF5797)(9)

Publications using AF5797

• Kentaro Nishida, Saho Bansho, Akiko Ikukawa, Teruyo Kubota, Akihiro Ohishi, Kazuki Nagasawa Expression profile of the zinc transporter ZnT3 in taste cells of rat circumvallate papillae and its role in zinc release, a potential mechanism for taste stimulation European Journal of Histochemistry 2022-11-10 [PMID: 36373349]
• N Shigemura, S Takai, F Hirose, R Yoshida, K Sanematsu, Y Ninomiya Expression of Renin-Angiotensin System Components in the Taste Organ of Mice Nutrients, 2019-09-19;11(9):. 2019-09-19 [PMID: 31546789] (IHC-Fr, Mouse)
• Kentaro Nishida, Yuka Nomura, Kanako Kawamori, Akihiro Ohishi, Kazuki Nagasawa ATP metabolizing enzymes ENPP1, 2 and 3 are localized in sensory neurons of rat dorsal root ganglion European Journal of Histochemistry 2018-04-09 [PMID: 29943954]
• W Yu, WG Hill, SC Robson, ML Zeidel Role of P2XReceptor in Mouse Voiding Function Sci Rep, 2018-01-30;8(1):1838. 2018-01-30 [PMID: 29382907] (IHC-Fr, Mouse)
• Kentaro Nishida, Teruyo Kubota, Saki Matsumoto, Junki Kato, Yu Watanabe, Atsuko Yamamoto et al. Expression of Prostatic Acid Phosphatase in Rat Circumvallate Papillae PLOS ONE 2016-06-27 [PMID: 27348306]
• Akihiro Ohishi, Kentaro Nishida, Yuri Yamanaka, Ai Miyata, Akiko Ikukawa, Miharu Yabu et al. Oxaliplatin Alters Expression of T1R2 Receptor and Sensitivity to Sweet Taste in Rats Biological & Pharmaceutical Bulletin 2016-04-05 [PMID: 27040630]
• Weiqun Yu, Warren G. Hill Lack of specificity shown by P2Y6 receptor antibodies Naunyn-Schmiedeberg's Archives of Pharmacology 2013-10-01 [PMID: 23793102]
• Weiqun Yu, Mark L. Zeidel, Warren G. Hill Cellular Expression Profile for Interstitial Cells of Cajal in Bladder - A Cell Often Misidentified as Myocyte or Myofibroblast PLoS ONE 2012-11-07 [PMID: 23145014] (Immunohistochemistry, Mouse)
• Yu W, Robson SC, Hill WG Expression and distribution of ectonucleotidases in mouse urinary bladder. PLoS ONE, 2011-04-14;6(4):e18704. 2011-04-14 [PMID: 21533188] (IHC-Fr, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ENTPD2
• Uniprot:
  • O55026()