CD39/ENTPD1 Antibody [Unconjugated]

Mouse splenocytes were stained with Mouse CD39/ENTPD1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4398, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

CD39/ENTPD1 was detected in perfusion fixed frozen sections of mouse liver using Mouse CD39/ENTPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4398) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD39/ENTPD1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1
  Thr38-Ile478
  Accession # Q921Q6
• Specificity: Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human CD39 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ENTPD1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39/ENTPD1 Antibody [Unconjugated]

• ADPase
• ATPDase
• ATP-diphosphatase
• CD39 antigen
• CD39
• CD39EC 3.6.1.5
• DKFZp686D194
• DKFZp686I093
• EC 3.6.1
• ecto-apyrase
• Ecto-ATP diphosphohydrolase 1
• Ecto-ATPase 1
• Ecto-ATPDase 1
• ectonucleoside triphosphate diphosphohydrolase 1
• ENTPD1
• FLJ40921
• FLJ40959
• Lymphoid cell activation antigen
• NTPD1
• NTPDase 1
• NTPDase-1

Background

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. Recombinant mouse NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (1), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (2). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (3). NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation (4). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (5).

1. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
2. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
3. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
4. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
5. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD39/ENTPD1 Antibody (AF4398)(5)

Publications using AF4398

• Vinícius Santos Alves, Stephanie Alexia Cristina Silva Santos, Raíssa Leite-Aguiar, Elaine Paiva-Pereira, Renata Rodrigues dos Dos Reis, Mariana L. Calazans et al. SARS-CoV-2 Spike protein alters microglial purinergic signaling Frontiers in Immunology 4/11/2023 [PMID: 37114062]
• A Badimon, HJ Strasburge, P Ayata, X Chen, A Nair, A Ikegami, P Hwang, AT Chan, SM Graves, JO Uweru, C Ledderose, MG Kutlu, MA Wheeler, A Kahan, M Ishikawa, YC Wang, YE Loh, JX Jiang, DJ Surmeier, SC Robson, WG Junger, R Sebra, ES Calipari, PJ Kenny, UB Eyo, M Colonna, FJ Quintana, H Wake, V Gradinaru, A Schaefer Negative feedback control of neuronal activity by microglia Nature, 2020-09-30;0(0):. 2020-09-30 [PMID: 32999463] (Western Blot, Mouse)
• LEB Savio, P de Andrade, SACS Santos, JC de Sousa, SDS Oliveira, RD Minshall, E Kurtenbach, Y Wu, MS Longhi, SC Robson, R Coutinho-S P2X7 receptor activation increases caveolin-1 expression and macrophage lipid raft formation boosting CD39 activity J. Cell. Sci., 2020-03-06;0(0):. 2020-03-06 [PMID: 32005701] (IHC, Mouse)
• Yu-Ru V. Shih, Mengqian Liu, Seong Keun Kwon, Masayuki Iida, Ya Gong, Nivedita Sangaj et al. Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss Science Advances 8/21/2019 [PMID: 31457100]
• M De Giorgi, K Enjyoji, G Jiang, E Csizmadia, S Mitsuhashi, RJ Gumina, RT Smolenski, SC Robson Complete deletion of Cd39 is atheroprotective in apolipoprotein E-deficient mice J. Lipid Res., 2017-05-09;0(0):. 2017-05-09 [PMID: 28487312] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ENTPD1
• Uniprot:
  • Q921Q6()