CD35 Antibody (8C12) - Chimeric - Azide and BSA Free

Immunohistochemistry: CD35 Antibody (8C12) - Chimeric [NBP2-52668] - Immunohistochemical staining of rat stomach using anti-Complement receptor 1 antibody NBP2-52668. Formalin fixed rat stomach slices were were stained with NBP2-52668 at 3 ug/ml.

• Reactivity: Mu
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: 8C12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD35 Antibody (8C12) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD35 Antibody (8C12) - Chimeric - Azide and BSA Free (NBP2-52668) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Purified murine CR1 protein.
• Specificity: Recognises an epitope on CD35 (CR1, 190 kDa), but not on CD21 (CR2, 145-150 kDa), which are alternative splice variants of primary Cr2 gene transcripts.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. This antibody recognises murine CD35, expressed on the majority of peripheral B-cells and peritoneal macrophages and upon fMet-Leu-Phe stimulation.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD35 Antibody (8C12) - Chimeric - Azide and BSA Free

• C3BR
• CD35
• complement component (3b/4b) receptor 1 (Knops blood group)
• CR1
• KN

Background

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CR1