CD300a/LMIR1 Antibody (MEM-260)

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Flow Cytometry: CD300A Antibody (MEM-260) [NB110-58726]- Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
MEM-260
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD300a/LMIR1 Antibody (MEM-260) Summary

Immunogen
HPB acute lymphoid leukemia cell line (NM_007261.2).
Specificity
This antibody (clone MEM-260) reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD300A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 2 ug/ml
  • CyTOF-ready
Application Notes
Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD300a/LMIR1 Antibody (MEM-260)

  • CD300a antigenCMRF35H9
  • CD300a molecule
  • CD300a
  • CLM8
  • CLM-8
  • CMRF35H leukocyte immunoglobulin-like receptor
  • CMRF-35H
  • CMRF35-H
  • CMRF35-H9
  • CMRF-35-H9CD300 antigen-like family member A
  • CMRF35HIRp60
  • CMRF35-like molecule 8
  • IGSF12
  • IGSF12NK inhibitory receptor
  • Immunoglobulin superfamily member 12
  • Inhibitory receptor protein 60
  • IRC1
  • IRC1/IRC2
  • IRC2
  • IRp60
  • leukocyte membrane antigen
  • LMIR1
  • MAIR-I
  • Mcipr1
  • NKRL
  • Pigr4

Background

CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-58726AF405
Alexa Fluor 488 Labeled NB110-58726AF488
Alexa Fluor 647 Labeled NB110-58726AF647
Alexa Fluor 700 Labeled NB110-58726AF700
Allophycocyanin Labeled NB110-58726APC
DyLight 350 Labeled NB110-58726UV
DyLight 405 Labeled NB110-58726V
DyLight 488 Labeled NB110-58726G
DyLight 680 Labeled NB110-58726FR
DyLight 755 Labeled NB110-58726IR
PE Labeled NB110-58726PE
PerCP Labeled NB110-58726PCP

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Bioinformatics

Gene Symbol CD300A
Entrez
Uniprot