CD30 Ligand/TNFSF8 Antibody (116615)

• Reactivity: Hu
• Applications: WB
• Clone: 116615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD30 Ligand/TNFSF8 Antibody (116615) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD30 Ligand/TNFSF8
  Gln63-Asp234
  Accession # P32971
• Specificity: Detects human CD30 Ligand/TNFSF8 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse CD30 Ligand is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF8
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD30 Ligand/TNFSF8 Antibody (116615)

• CD153 antigen
• CD153
• CD30 antigen ligand
• CD30 Ligand
• CD30L
• CD30-L
• CD30LCD30 ligand
• CD30LGMGC138144
• TNFSF8
• tumor necrosis factor (ligand) superfamily, member 8
• tumor necrosis factor ligand superfamily member 8

Background

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells. The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. CD30 is also expressed on different non-Hodgkin's lymphomas, virus-infected T and B cells, and on normal T and B cells after activation. Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4⁺/CD8⁺ thymocytes that co‑express CD45RO and IL-4 receptor. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. CD30 can act as a co‑stimulatory molecule in thymic negative selection and may also play a critical role in the pathophysiology of Hodgkin's disease and other CD30⁺ lymphomas. Human and mouse CD30 ligand cDNAs share 70% sequence homology.

1. Brunangelo, F. et al. (1995) Blood 85:1.
2. Gruss, H.-J. and F. Herrmann (1996) Leukemia and Lymphoma 20:397.
3. Chiarle, R. et al. (1999) J. Immunol. 163:194.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD30 Ligand/TNFSF8 Antibody (MAB774)(1)

Publication using MAB774

• Simhadri VL, Hansen HP, Simhadri VR, Reiners KS, Bessler M, Engert A, von Strandmann EP A novel role for reciprocal CD30-CD30L signaling in the cross-talk between natural killer and dendritic cells. Biol. Chem., 2012-01-01;393(1):101-6. 2012-01-01 [PMID: 22628304] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TNFSF8
• Uniprot:
  • P32971()