CD3 epsilon Antibody

Images

 
Western Blot: CD3 epsilon Antibody [NB100-2000] - Western blot analysis of CD3e in the Jurket cell lysate with the anti-CD3 epsilon antibody
Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NB100-2000] - shows a positive cell membrane staining observed in formalin-fixed human tonsil tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD3 epsilon Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 156-168 of Human CD3, Epsilon chain.
Localization
Type I membrane protein.
Specificity
CD3
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD3E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • Immunohistochemistry 5.0-10.0 ug/ml
  • Immunohistochemistry-Paraffin 5.0-10.0 ug/ml
Application Notes
Western blot: Predicted molecular weight: 23 kDa. Sections should be boiled in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Tonsil Lysate (NB820-59272)
Publications
Read Publication using NB100-2000.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD3 epsilon Antibody

  • CD3 epsilon
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • FLJ18683
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 epsilon Antibody (NB100-2000)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD3E
Entrez
Uniprot