CD3 epsilon Antibody (E272)

Product Discontinued

CD3 epsilon Antibody (E272) Summary

• Immunogen: A synthetic peptide corresponding to residues in C-terminal cytoplasmic domain of human CD-3 epsilon chain was used as immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD3E
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:50
  • Western Blot 1:100-1:2000
• Application Notes: This product is useful for: Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 23kDa.
• Control:

  CD3 epsilon Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for CD3 epsilon Antibody (E272)

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD3 epsilon Antibody (NB110-55697)(2)

Publications using NB110-55697

• Schalper KA, Carvajal-Hausdorf D, McLaughlin J et al. Differential expression and significance of PD-L1, IDO-1 and B7-H4 in human lung cancer Clin. Cancer Res. 2016-07-20 [PMID: 27440266]
• Schalper KA, Brown J, Carvajal-Hausdorf D et al. Objective Measurement and Clinical Significance of TILs in Non-Small Cell Lung Cancer J. Natl. Cancer Inst. 2015-03-01 [PMID: 25650315] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CD3E
• Entrez:
  • 916(Human)
• Uniprot:
  • P07766()