CD3 epsilon Antibody (C363.29B (YCD3-1)) [Allophycocyanin]

Flow Cytometry: CD3 epsilon Antibody (C363.29B (YCD3-1)) [Allophycocyanin] [NBP1-26689] - Analysis of BALB/c splenocytes by multiple stainnig.

Product Discontinued

CD3 epsilon Antibody (C363.29B (YCD3-1)) [Allophycocyanin] Summary

• Immunogen: IL-4 producing Th2 cell lines including D10
• Specificity: Mouse CD3 epsilon (Mr 25 kD)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD3E
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
• Application Notes: This antibody is useful for Immunoprecipitation, Flow cytometry / FACS analysis, Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and a stabilizing agent
• Preservative: Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD3 epsilon Antibody (C363.29B (YCD3-1)) [Allophycocyanin]

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3E