CD3 epsilon Antibody (C363.29B (YCD3-1))

Images

 
Flow Cytometry: CD3 epsilon Antibody (C363.29B (YCD3-1)) [NBP1-26685] - Analysis using the PE conjugate of NBP1-26685. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD3 epsilon Antibody (C363.29B (YCD3-1)) [NBP1-26685] - Analysis using the Allophycocyanin conjugate of NBP1-26685. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD3 epsilon Antibody (C363.29B (YCD3-1)) [NBP1-26685] - Analysis using the Biotin conjugate of NBP1-26685. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD3 epsilon Antibody (C363.29B (YCD3-1)) [NBP1-26685] - Analysis using the Cy5 conjugate of NBP1-26685. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD3 epsilon Antibody (C363.29B (YCD3-1)) [NBP1-26685] - Analysis using the FITC conjugate of NBP1-26685. Double staining of 3 ug/1^6 Cells from BALB/c mesenteric lymph nodes with anti-mouse CD3 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, In vitro, IP, BA
Clone
C363.29B (YCD3-1)
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates

CD3 epsilon Antibody (C363.29B (YCD3-1)) Summary

Immunogen
IL-4 producing Th2 cell lines including D10
Specificity
Mouse CD3 epsilon (Mr 25 kD)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD3E
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Bioactivity
Application Notes
Flow Cytometry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro depletion of CD3+ cells, In vitro activation of T cells (accessory cells must be provided)
Publications
Read Publications using NBP1-26685.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD3 epsilon Antibody (C363.29B (YCD3-1))

  • CD3 epsilon
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • FLJ18683
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains.(1, 2) CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.(1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naive T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.(1, 3, 4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.(1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 epsilon Antibody (NBP1-26685)(10)


Showing Publications 1 - 10 of 10.
Publications using NBP1-26685 Applications Species
WhitelJL, Nicholls SM, Shimeld C et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies J Histochem Cytochem 1995 Mar [PMID: 7868861]

Details:
This citation used the Biotin version of this antibody.
Portoles P, Rojo J, Golby A et al. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation J Immunol 1989 Jun [PMID: 2470817]

Details:
This citation used the Biotin version of this antibody.
Dianzani U, Luqman M, Rojo J et al. Molecular associations on the T cell surface correlate with immunological memory Eur J Immunol 1990 Oct [PMID: 1978709]

Details:
This citation used the Biotin version of this antibody.
Ernst DN, Hobbs MV, Torbett BE et al. Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice J Immunol [PMID: 1974562]

Details:
Used the Biotin form of this antibody.
Luqman M, Greenbaum L, Lu D et al. Differential effect of interleukin 1 on naive and memory CD4+ T cells Eur J Immunol 1992 Jan [PMID: 1346115]

Details:
This citation used the Biotin version of this antibody.
Luqman M, Greenbaum L, Lu D et al. Differential effect of interleukin 1 on naive and memory CD4+ T cells Eur J Immunol

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-26687).
Dianzani U, Luqman M, Rojo J et al. Molecular associations on the T cell surface correlate with immunological memory Eur J Immunol

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-26687).
Ernst DN, Hobbs MV, Torbett BE et al. Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice J Immunol

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-26687).
Portoles P, Rojo J, Golby A et al. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation J Immunol

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-26687).
Whitel JL, Nicholls SM, Shimeld C et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies J Histochem Cytochem

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-26687).

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Bioinformatics

Gene Symbol CD3E
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