CD3 epsilon Antibody (1033713) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clone: 1033713
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

CD3 epsilon Antibody (1033713) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human CD3 epsilon in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 epsilon Antibody (1033713) [Alexa Fluor™ Plus 594]

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 epsilon (T-Cell Surface Glycoprotein CD3 Epsilon) is a type I transmembrane protein that belongs to the Ig (Immunoglobulin) superfamily. Human CD3 epsilon consists of 104 amino acid (aa) extracellular domain, 26 aa transmembrane domain and 55 aa cytoplasmic domain (1). With the extracellular domain, human CD3 epsilon shares 44.2% and 49% homology with mouse and rat CD3 epsilon respectively. There are four CD3 proteins: CD3δ, CD3 epsilon , CD3 gamma , and CD3 zeta . All CD3 proteins contain ITAM (Immunoreceptor Tyrosine-based Activation Motifs) in the cytoplasmic tail, which becomes phosphorylated by Src family protein tyrosine kinases LCK and FYN upon TCR engagement (2). CD3 proteins form heterodimers of CD3δ/CD3 epsilon and CD3 gamma /CD3 epsilon , which bind to TCR and form trimeric TCR alpha /CD3 epsilon /CD3 gamma and TCR beta /CD3 gamma /CD3 epsilon . The resulting heterohexamer further associates with CD3 zeta homodimer and forms TCR/CD3 signaling complex (3, 4). Similar complex is also formed with TCR gamma and TCRδ (5). CD3 epsilon plays essential role in adaptive immune response. Mutations in CD3 epsilon lead to SCID (severe combined immunodeficiency) (6).

1. Gold, D.P. et al. (1986) Nature 321:431.
2. Barber, E.K. et al. (1989) Proc. Nat. Acad. Sci. U.S.A. 86:3277.
3. Manolios, N. et al. (1991) EMBO J 10:1643.
4. Dietrich, J. et al. (1999) Eur J Immunol 29:1719.
5. Pan, Q. et al. (2006) Mol Immunol 43:1741.
6. Soudais, C. et al. (1993) Nat. Genet. 3:77.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.