CD3 delta Antibody (JJ08-97)

Western Blot: CD3 delta Antibody (JJ08-97) [NBP2-67882] - Analysis of CD3D on Jurkat cells lysates using anti-CD3D antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: CD3 delta Antibody (JJ08-97) [NBP2-67882] - Staining CD3D in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CD3 delta Antibody (JJ08-97) [NBP2-67882] - Analysis of paraffin-embedded human tonsil tissue using anti-CD3D antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: CD3 delta Antibody (JJ08-97) [NBP2-67882] - Staining CD3D in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CD3 delta Antibody (JJ08-97) [NBP2-67882] - Staining CD3D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CD3 delta Antibody (JJ08-97) [NBP2-67882] - Analysis of paraffin-embedded human kidney tissue using anti-CD3D antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD3 delta Antibody (JJ08-97) [NBP2-67882] - Analysis of paraffin-embedded human spleen tissue using anti-CD3D antibody. Counter stained with hematoxylin.

Product Discontinued

CD3 delta Antibody (JJ08-97) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD3D
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 1%BSA, 40%Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD3 delta Antibody (JJ08-97)

• CD3 antigen, delta subunit
• CD3 delta
• CD3d antigen
• CD3d antigen, delta polypeptide (TiT3 complex)
• CD3d molecule, delta (CD3-TCR complex)
• CD3D
• CD3-DELTA
• T3DOKT3, delta chain
• T-cell receptor T3 delta chain
• T-cell surface glycoprotein CD3 delta chain

Background

CD3 delta is encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3D