CD3 delta Antibody (1033614) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 1033614
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

CD3 delta Antibody (1033614) [Alexa Fluor® 532] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived human CD3 delta/CD3d
  Phe22-Ala105
  Accession # P04234
• Specificity: Detects human CD3 delta/CD3d in ELISA and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 delta Antibody (1033614) [Alexa Fluor® 532]

• CD3 antigen, delta subunit
• CD3 delta
• CD3d antigen
• CD3d antigen, delta polypeptide (TiT3 complex)
• CD3d molecule, delta (CD3-TCR complex)
• CD3D
• CD3-DELTA
• T3DOKT3, delta chain
• T-cell receptor T3 delta chain
• T-cell surface glycoprotein CD3 delta chain

Background

CD3d, also known as CD3 delta and T3D, is a single-pass type I membrane protein. CD3 delta/CD3d is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction (1). The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T cells (2). CD3 delta/CD3d contains an 84 amino acid extracellular domain, a 21 amino acid transmembrane domain, and a 45 amino acid cytoplasmic domain. Human CD3 delta/CD3d shares 89%, 61%, and 57% sequence identity in its extracellular domain with its cynomolgus monkey, rat, and mouse homologs, respectively. CD3 delta/CD3d, like other subunit members CD3G and CD3E of the TCR-CD3 complex, contains an extracellular Ig domain and a single immunoreceptor tyrosine-based activation motif (3, 4). Defects in CD3 delta/CD3d cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels (5, 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.