CD277/BTN3A1 Antibody [Alexa Fluor® 532]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

CD277/BTN3A1 Antibody [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human BTN3A2
  Gln30-Trp248
  Accession # P78410
• Specificity: Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region (aa 30-248) with one V-type Ig-like domain (aa 30-134), and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. One possesses an alternative start site 43 aa upstream of the standard site, a second contains a five aa substitution for aa 1-28, and a third shows a 24 aa substitution for aa 307-334. A rodent counterpart to BTN3A2 has not been reported. Human BTN3A1 (Butyrophilin subfamily 3 member A1; also BTF5) over aa 30-248 shares 95.4% aa identity with human NTN3A2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.