BTN3A1/2/3 Antibody [Unconjugated]

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BTN3A1/2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7136) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BTN3A1/2/3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

BTN3A1/2/3 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human BTN3A2
  Gln30-Trp248
  Accession # P78410
• Specificity: Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: BTN3A2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTN3A1/2/3 Antibody [Unconjugated]

• BT3.1
• BTF5
• BTN3.1
• BTN3A1/2/3
• butyrophilin subfamily 3 member A1
• CD277
• dJ45P21.3 (butyrophilin, subfamily 3, member A1)

Background

BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region (aa 30-248) with one V-type Ig-like domain (aa 30-134), and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. One possesses an alternative start site 43 aa upstream of the standard site, a second contains a five aa substitution for aa 1-28, and a third shows a 24 aa substitution for aa 307-334. A rodent counterpart to BTN3A2 has not been reported. Human BTN3A1 (Butyrophilin subfamily 3 member A1; also BTF5) over aa 30-248 shares 95.4% aa identity with human NTN3A2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BTN3A2
• Uniprot:
  • P78410(Human)