CD27 Ligand/TNFSF7/CD70 Antibody (TNFS7/1026) - Azide and BSA Free

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC, ICC/IF
• Clone: TNFS7/1026
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD27 Ligand/TNFSF7/CD70 Antibody (TNFS7/1026) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44546).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human CD27 Ligand/TNFSF7/CD70 protein (Uniprot: P32970)
• Localization: Cell Surface
• Marker: Activated T- & B-Lymphocyte Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD70
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5 - 1 ug/ml
• Application Notes: ELISA: For coating, order Ab without BSA.
  Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD27 Ligand/TNFSF7/CD70 Antibody (TNFS7/1026) - Azide and BSA Free

• CD27 Ligand
• CD27-L
• CD27LCD27 ligand
• CD27LG
• CD70 molecule
• CD70
• Ki-24 antigen
• surface antigen CD70
• TNFSF7
• TNFSF7CD70 antigen
• tumor necrosis factor (ligand) superfamily, member 7
• Tumor necrosis factor ligand superfamily member 7

Background

CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD70