CD27 Ligand/TNFSF7/CD70 Antibody (BU69)

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC, IP, ICC/IF
• Clone: BU69
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD27 Ligand/TNFSF7/CD70 Antibody (BU69) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.(NBP3-11586)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Clone BU69 was used by HLDA to establish CD designation.
• Immunogen: Human WM-1 (Waldenstrom s macroglobulinemia) cell line
• Localization: Cell Surface
• Marker: Activated T- & B-Lymphocyte Marker
• Specificity: It recognizes a protein of 30kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This monoclonal antibody blocks the interaction between CD27 and CD70, and has been shown to inhibit T cell proliferation induced by dendritic cells.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD70
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/500 ug protein lysate
• Application Notes: ELISA: For coating, order Ab without BSA.
  Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
  Inhibits T cell proliferation induced by dendritic cells: Order Ab without Azide.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD27 Ligand/TNFSF7/CD70 Antibody (BU69)

• CD27 Ligand
• CD27-L
• CD27LCD27 ligand
• CD27LG
• CD70 molecule
• CD70
• Ki-24 antigen
• surface antigen CD70
• TNFSF7
• TNFSF7CD70 antigen
• tumor necrosis factor (ligand) superfamily, member 7
• Tumor necrosis factor ligand superfamily member 7

Background

CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD70
• Uniprot:
  • P32970()
  • P32970()