CD27 Ligand/TNFSF7/CD70 Antibody (118510) [Unconjugated]

In the presence of sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484), Recom­binant Mouse CD27 Ligand/ TNFSF7 (Catalog # 783-CL) stimulates proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, prolif­eration elicited by Recombinant Mouse CD27 Ligand/TNFSF7 (10 µg/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse CD27 Ligand/ TNFSF7 Monoclonal Antibody (Catalog # MAB783). The ND50 is typically 0.5-2 µg/mL.

• Reactivity: Mu
• Applications: B/N, ELISA(Cap)
• Clone: 118510
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CD27 Ligand/TNFSF7/CD70 Antibody (118510) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CD27 Ligand/TNFSF7
  Gln47-Pro195
  Accession # O55237
• Specificity: Detects mouse CD27 Ligand/TNFSF7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD30 Ligand or rhCD40 Ligand is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CD70
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.5-2 ug/mL
• Application Notes: ELISA Detection: Mouse CD27 Ligand/TNFSF7 Biotinylated Affinity Purified PAb (Catalog number BAF783)
  Standard: Recombinant Mouse CD27 Ligand/TNFSF7 (Catalog number 783-CL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD27 Ligand/TNFSF7/CD70 Antibody (118510) [Unconjugated]

• CD27 Ligand
• CD27-L
• CD27LCD27 ligand
• CD27LG
• CD70 molecule
• CD70
• Ki-24 antigen
• surface antigen CD70
• TNFSF7
• TNFSF7CD70 antigen
• tumor necrosis factor (ligand) superfamily, member 7
• Tumor necrosis factor ligand superfamily member 7

Background

CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). Mouse CD27L cDNA encodes a 195 amino acid (aa) residue protein with a 23 aa N-terminal cytoplasmic domain, a 21 transmembrane domain and a 151 aa C‑terminal extracellular domain. Mouse and human CD27L share approximated 56% aa sequence identity. By analogy to other TNFSF ligands, CD27L is expected to exist as non-covalent homotrimers. The expression of CD27L is highly regulated at the transcription and posttranslational level (3). CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. Although CD27L expression is not detected on human dendritic cells (DC), membrane expression on mature mouse DCs has been reported. CD27L expression is also present in the thymus medulla in both human and mouse. CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on natural killer (NK) cells and subsets of T and B cells (2, 4, 5). Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (2). Ligation of CD27 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses (5, 6). On NK cells, ligation of CD27 induces proliferation and IFN-gamma production (4).

1. Hitzen, R.Q. et al. (1994) Immunol. Today 15:307.
2. Croft, M. (2003) Nature Reviews Immunol. 3:609.
3. Tesselaar, K. et al. (2003) J. Immunol. 169:33.
4. Takeda, K. et al. (2000) J. Immunol. 164:1741.
5. Kobata, T.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11249.
6. Raman, V.S. et al. (2003) J. Immunol. 171:5876.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD70
• Uniprot:
  • O55237()