CD24 Antibody (M1/69)

Product Discontinued

CD24 Antibody (M1/69) Summary

• Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD24
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells in 100 ul
• Application Notes: Western blotting, in vitro induction of thymocyte maturation, complement-mediated cytotoxicity, and IHC (Frozen and Paraffin sections) use reported in literature.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24277698)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD24 Antibody (M1/69)

• CD 24
• CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• CD24 antigen
• CD24 molecule
• CD24
• CD24A
• CD24Asignal transducer CD24
• FLJ22950
• FLJ43543
• MGC75043
• Small cell lung carcinoma cluster 4 antigen

Background

CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD24 Antibody (NB100-77388)(3)

Publications using NB100-77388

• Koren S, Reavie L, do Couto JP et al. PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours Nature (Mouse)
  
  Details:
  This publication used the Alexa Fluor (R) 700 conjugated form of this antibody (Cat# NB100-77388AF700).
• Koren S, Reavie L, do Couto JP et al. PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours Nature 2015-08-13 [PMID: 26266975]
• Volonte A, Di Tomaso T, Spinelli M et al. Cancer-Initiating Cells from Colorectal Cancer Patients Escape from T Cell-Mediated Immunosurveillance In Vitro through Membrane-Bound IL-4. J Immunol. 2013-11-25 [PMID: 24277698] (FLOW, Human)

Bioinformatics

• Gene Symbol: CD24
• Entrez:
  • 12484(Mouse)
• Uniprot:
  • P24807()