CD200/OX2 Antibody (325531) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: B/N, ELISA(Cap)
• Clone: 325531
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

CD200/OX2 Antibody (325531) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD200
  Gln31-Gly232
  Accession # P41217.3
• Specificity: Detects human CD200 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse CD200 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD200
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • ELISA Capture (Matched Antibody Pair)
  • Neutralization
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CD200/OX2 Antibody (325531) [CoraFluor™ 1]

• antigen identified by monoclonal MRC OX-2
• CD200 antigenMOX1
• CD200 molecule
• CD200
• MOX1
• MOX2
• MOX2MRC
• MRC OX-2 antigen
• MRC
• OX-2 membrane glycoprotein
• OX-2

Background

CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. It is a monomorphic determinant expressed on thymocytes, brain, follicular dendritic cells in all lymphoid organs, endothelium and at low levels on some smooth muscle and B lymphocytes. Recent studies have suggested that the CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD200