CD2 Antibody [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

CD2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD2
  Arg23-Ser203
  Accession # P08920
• Specificity: Detects mouse CD2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human CD2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD2 Antibody [Alexa Fluor® 488]

• CD2 antigen (p50), sheep red blood cell receptor
• CD2 antigen
• CD2 molecule
• CD2
• Erythrocyte receptor
• FLJ46032
• LFA-2
• LFA-3 receptor
• lymphocyte-function antigen-2
• Rosette receptor
• SRBC
• T11
• T-cell surface antigen CD2
• T-cell surface antigen T11/Leu-5

Background

Mouse CD2, also known as LFA-2 and LFA-3 Receptor, is a 55‑66 kDa, variably glycosylated type I transmembrane (TM) protein belonging to the CD2 receptor subset of the Ig superfamily. It is synthesized as a 344 amino acid (aa) precursor that contains a 22 aa signal sequence, a 181 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a proline rich (20%) 115 aa cytoplasmic region. CD2 is found on T and NK cells and binds CD48 in rodents and CD58 in humans. The ECD of mouse CD2 shares 73%, 50% and 44% aa identity with the CD2 ECD in rat, human and equine, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.