CD2 Antibody (I/35A)

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Product Details

Summary
Reactivity Gt, ShSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
Clone
I/35A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD2 Antibody (I/35A) Summary

Immunogen
Sheep T cell blasts
Specificity
NB100-65316 recognizes ovine CD2, a 55kD cell surface antigen. Ovine CD2 is expressed by peripheral T cells expressing CD4 or CD8, but not by the CD4/CD8 negative subset, which comprises approximately 20% of cells in peripheral blood. In the thymus most cells express low levels of CD2. In immunohistochemical studies tissue macrophages in lymph nodes are found to be CD2 +ve. NB100-65316 blocks sheep autologous E rosetting.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Reacts with Sheep. Cross reacts with Goat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD2 Antibody (I/35A)

  • CD2 antigen (p50), sheep red blood cell receptor
  • CD2 antigen
  • CD2 molecule
  • CD2
  • Erythrocyte receptor
  • FLJ46032
  • LFA-2
  • LFA-3 receptor
  • lymphocyte-function antigen-2
  • Rosette receptor
  • SRBC
  • T11
  • T-cell surface antigen CD2
  • T-cell surface antigen T11/Leu-5

Background

CD2 is a surface antigen of the human T lymphocyte lineage that is expressed on all peripheral blood T cells. It is one of the earliest T cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types. CD2 is implicated in the triggering of T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for the identification of lymphomas and leukemias of T cell origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CD2 Antibody (NB100-65316) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-65316AF405
Alexa Fluor 488 Labeled NB100-65316AF488
Alexa Fluor 647 Labeled NB100-65316AF647
Alexa Fluor 700 Labeled NB100-65316AF700
Allophycocyanin Labeled NB100-65316APC
DyLight 350 Labeled NB100-65316UV
DyLight 405 Labeled NB100-65316V
DyLight 488 Labeled NB100-65316G
DyLight 680 Labeled NB100-65316FR
DyLight 755 Labeled NB100-65316IR
PE Labeled NB100-65316PE
PerCP Labeled NB100-65316PCP

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Bioinformatics

Gene Symbol CD2
Entrez