CD177 Antibody (1171A) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G APC‑conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rabbit Anti-Mouse CD177 Monoclonal Antibody (Catalog # MAB8186) or (B) Normal Rabbit IgG Control (Catalog #AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

• Reactivity: Mu
• Applications: Flow
• Clone: 1171A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD177 Antibody (1171A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant mouse CD177
  Accession # Q8R2S8
• Specificity: Detects mouse CD177 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD177
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD177 Antibody (1171A) [Unconjugated]

• CD177 antigenNB1 GP
• CD177 molecule
• CD177
• HNA-2a
• HNA2Acell surface receptor
• Human neutrophil alloantigen 2a
• NB1 GP
• NB1PRV-1
• polycythemia rubra vera 1
• Polycythemia rubra vera protein 1
• PRV1
• PRV1NB1 glycoprotein

Background

CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
2. Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
3. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
4. Klippel, S. et al. (2002) Blood 100:2441.
5. Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
6. Temerinac, S. et al. (2000) Blood 95:2569.
7. Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
8. Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
9. Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
10. von Vietinghoff, S. et al. (2007) Blood 109:4487.
11. Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
12. van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD177 Antibody (MAB8186)(2)

Publications using MAB8186

• Pallavi, R;Gatti, E;Durfort, T;Stendardo, M;Ravasio, R;Leonardi, T;Falvo, P;Duso, BA;Punzi, S;Xieraili, A;Polazzi, A;Verrelli, D;Trastulli, D;Ronzoni, S;Frascolla, S;Perticari, G;Elgendy, M;Varasi, M;Colombo, E;Giorgio, M;Lanfrancone, L;Minucci, S;Mazzarella, L;Pelicci, PG; Caloric restriction leads to druggable LSD1-dependent cancer stem cells expansion Nature communications 2024-01-27 [PMID: 38280853] (Flow Cytometry, Mouse)
• N Wu, XN Nguyen, L Wang, R Appourchau, C Zhang, B Panthu, H Gruffat, C Journo, S Alais, J Qin, N Zhang, K Tartour, F Catez, R Mahieux, T Ohlmann, M Liu, B Du, A Cimarelli The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation PLoS Pathog., 2019-10-10;15(10):e1008093. 2019-10-10 [PMID: 31600344]

Bioinformatics

• Gene Symbol: CD177
• Uniprot:
  • Q8R2S8()