CD177 Antibody (1171A) [Alexa Fluor™ Plus 405]

• Reactivity: Mu
• Clone: 1171A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 405

CD177 Antibody (1171A) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects mouse CD177 in direct ELISAs.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD177 Antibody (1171A) [Alexa Fluor™ Plus 405]

• CD177 antigenNB1 GP
• CD177 molecule
• CD177
• HNA-2a
• HNA2Acell surface receptor
• Human neutrophil alloantigen 2a
• NB1 GP
• NB1PRV-1
• polycythemia rubra vera 1
• Polycythemia rubra vera protein 1
• PRV1
• PRV1NB1 glycoprotein

Background

CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
2. Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
3. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
4. Klippel, S. et al. (2002) Blood 100:2441.
5. Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
6. Temerinac, S. et al. (2000) Blood 95:2569.
7. Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
8. Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
9. Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
10. von Vietinghoff, S. et al. (2007) Blood 109:4487.
11. Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
12. van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.