CD161/NK1.1 Antibody (694370) [Alexa Fluor® 488]

Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rat Anti-Mouse CD161/NK1.1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7614G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 694370
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

CD161/NK1.1 Antibody (694370) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD161/NK1.1
  Val62-Ser220
  Accession # P27814
• Specificity: Detects mouse CD161/NK1.1 expressed specifically in the C57/B6 mouse strain. No detection of CD161/NK1.1 was observed in the BALB/c mouse strain. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) KLRB-1B is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: KLRB1C
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD161/NK1.1 Antibody (694370) [Alexa Fluor® 488]

• CD161 antigen
• CD161
• CD161/NK1.1
• CD161c
• CLEC5BC-type lectin domain family 5 member B
• HNKR-P1a
• killer cell lectin-like receptor subfamily B member 1
• killer cell lectin-like receptor subfamily B, member 1
• Klrb1c
• Ly55c
• Ly-59
• Natural killer cell surface protein P1A
• NKR-P1
• NKR-P1AMGC138614
• NKRP1ANKR

Background

KLRB-1C, also known as NK1.1, CD161c, Ly-55, and NKR-P1c, is a 28 kDa type 2 transmembrane protein in the killer cell lectin-like receptor family. CD161 is expressed as a disulfide-linked homodimer on the surface of NK cells and subpopulations of NKT, CD4⁺, CD8⁺, and gamma /δ T cells. Its cross-linking on NK cells induces cytolytic activity, and on CD4 cells it promotes IgE production and the expansion of Th2 responses. Mature mouse CD161 consists of a 45 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 157 aa extracellular domain with one C-type lectin domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD161/NK1.1 Antibody (FAB7614G)(1)

Publication using FAB7614G

• GD García Nor, CL Ly, DA Cuzzone, RP Kataru, GE Hespe, JS Torrisi, JJ Huang, JC Gardenier, IL Savetsky, MD Nitti, JZ Yu, S Rehal, BJ Mehrara CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema Nat Commun, 2018-05-17;9(1):1970. 2018-05-17 [PMID: 29773802] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: KLRB1C
• Uniprot:
  • P27814()