Rat CD109 ELISA Kit (Colorimetric)

ELISA: Rat CD109 ELISA Kit (Colorimetric) [NBP2-75129] - Samples were spiked with high concentrations of Rat CD109 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat CD109 ELISA Kit (Colorimetric) [NBP2-75129] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Rat CD109 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat CD109 in samples. No significant cross-reactivity or interference between Rat CD109 and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.16%
• Intra-Assay: CV% < 4.25%
• Spike Recovery: 85-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CD109

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat CD109 ELISA Kit (Colorimetric)

• 150 kDa TGF-beta-1-binding protein
• activated T-cell marker CD109
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7
• CD109 antigen (Gov platelet alloantigens)
• CD109 antigen
• CD109 molecule
• CD109
• CPAMD7
• CPAMD7r150
• DKFZp762L1111
• FLJ38569
• FLJ41966
• Gov platelet alloantigens
• p180
• Platelet-specific Gov antigen
• RP11-525G3.1

Background

CD109 is a GPI-linked cell surface antigen expressed by CD34+ acute myeloid leukemia cell lines, T-cell lines, activated T lymphoblasts, endothelial cells, and activated platelets (Lin et al., 2002 [PubMed 11861284]). In addition, the platelet-specific Gov antigen system, implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and posttransfusion purpura, is carried by CD109 (Kelton et al., 1990 [PubMed 2346781]; Lin et al., 2002 [PubMed 11861284]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CD109 ELISA Kit (NBP2-75129). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CD109