CCR2 Overexpression Lysate (Native)

Images

 
Western Blot: CCR2 Overexpression Lysate (Adult Normal) [NBP2-09417] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCR2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CCR2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001116868. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CCR2, mRNA.
Gene
CCR2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
40.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CCR2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CCR2 Overexpression Lysate (Native)

  • C-C chemokine receptor type 2
  • C-C CKR-2
  • CC-CKR-2CKR2B
  • CCR2
  • CCR-2
  • CCR2A
  • CCR2B
  • CD192 antigen
  • CD192
  • chemokine (C-C motif) receptor 2
  • CKR2
  • CKR2A
  • CMKBR2MGC111760
  • FLJ78302
  • MCP-1 receptor
  • MCP-1-RMGC103828
  • MGC168006
  • Monocyte chemoattractant protein 1 receptor
  • monocyte chemotactic protein 1 receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB182
Species: Hu
Applications: Flow, CyTOF-ready, ICC, Neut
MAB145
Species: Hu
Applications: Flow, CyTOF-ready
MAB155
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
MAB172
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
MAB330
Species: Hu
Applications: Flow, IHC, CyTOF-reported, Neut
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
MAB160
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
NBP1-76949
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P
MAB1567
Species: Hu
Applications: Flow, CyTOF-reported
MAB2005
Species: Hu
Applications: WB, Flow, CyTOF-ready
AF7177
Species: Hu
Applications: WB, ICC
NBP2-37856
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-86564
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-09417
Species: Hu
Applications: WB

Publications for CCR2 Lysate (NBP2-09417) (0)

There are no publications for CCR2 Lysate (NBP2-09417).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CCR2 Lysate (NBP2-09417) (0)

There are no reviews for CCR2 Lysate (NBP2-09417). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCR2 Lysate (NBP2-09417) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CCR2 Products

Bioinformatics Tool for CCR2 Lysate (NBP2-09417)

Discover related pathways, diseases and genes to CCR2 Lysate (NBP2-09417). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CCR2 Lysate (NBP2-09417)

Discover more about diseases related to CCR2 Lysate (NBP2-09417).
 

Pathways for CCR2 Lysate (NBP2-09417)

View related products by pathway.

PTMs for CCR2 Lysate (NBP2-09417)

Learn more about PTMs related to CCR2 Lysate (NBP2-09417).

Blogs on CCR2.

CCR2 or CD192
CCR2 is a receptor for several monocyte chemoattractant proteins (MCP1, MCP3, MCP4) that specifically govern monocyte chemotaxis. CCR2 transduces its downstream signals through increasing intracellular calcium ion levels. For example, MCP1 regulate...  Read full blog post.

CCR2: Affecting Autoimmunity via MCP1 interactions
CCR2, also known as CD192 (cluster of differentiation 192), is a chemokine receptor and is expressed by monocytes, activated T cells, B cells and natural killer cells. This protein is encoded by CCR2 gene in humans. CCR2 gene encodes two protein isofo...  Read full blog post.

MCP1: One Chemoattractant that's Hard to Resist
Monocyte Chemoattractant Protein (MCP1) is a potent monocyte attractant, is a member of the CC chemokine subfamily. MCP1 exerts its effects through binding to G-protein-coupled receptors on the surface of leukocytes targeted for activation and migrati...  Read full blog post.

Customers Who Bought This Also Bought

CCR2 Blocking Peptide
NBP1-48337PEP
CCR2 Antibody
NBP1-48337

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCR2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CCR2
Entrez