Recombinant Mouse CCL9/10/MIP-1 gamma Protein

SDS-Page: Mouse CCL9/10/MIP-1 gamma Protein [NBP2-35086]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CCL9/10/MIP-1 gamma Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 101 amino acids corresponding to CCL9/10/MIP-1 gamma Source: E. coli

  Uniprot ID: P51670

  Amino Acid Sequence: QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q

• Details of Functionality: Macrophage Marker Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL9/10
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL9/10/MIP-1 gamma as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 11.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CCL9/10/MIP-1 gamma Protein

• C-C motif chemokine 9
• CCF18
• CCL9/10
• chemokine (C-C motif) ligand 9
• macrophage inflammatory protein 1-gamma
• macrophage inflammatory protein-related protein 2
• MIP1 gamma
• MIP-1 gamma
• MRP-2
• SCYA10
• Scya9
• small inducible cytokine A10
• small inducible cytokine A9

Background

Murine MIP-1 gamma (CCL9/10) is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine MIP-1 gamma (CCL9/10) is 75% amino acid identical to rat CCL9/10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL9/10
• Uniprot:
  • P51670()
  • P51670()
  • P51670()