Recombinant Mouse CCL8/MCP-2 Protein

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CCL8/MCP-2 Protein Summary

• Description: A single, non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL8/MCP-2 Source: E. coli

  Uniprot ID: Q9Z121

  Amino Acid Sequence: GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP

• Details of Functionality: CCL8/MCP-2 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL8
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL8/MCP-2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 8.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CCL8/MCP-2 Protein

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• MCP-2/CCL8
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

Murine CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family and is encoded by the gene CCL8 in mouse. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic fuction for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Review for CCL8/MCP-2 Recombinant Protein (NBP2-35159) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Katherine Boule Immunoassay Standard 09/21/2018

Summary

• Application: Immunoassay Standard
• Lot: F22408041

Bioinformatics

• Gene Symbol: CCL8
• Uniprot:
  • Q9Z121()
  • Q9Z121()
  • Q9Z121()