Recombinant Mouse CCL7/MCP-3/MARC Protein

SDS-Page: Mouse CCL7/MCP-3/MARC Protein [NBP2-35161]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CCL7/MCP-3/MARC Protein Summary

• Description: A single, non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL7/MCP-3/MARC Source: E. coli

  Uniprot ID: Q03366

  Amino Acid Sequence: QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP

• Details of Functionality: MCP3 Protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL7
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL7/MCP-3/MARC as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 8.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 2x PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CCL7/MCP-3/MARC Protein

• C-C motif chemokine 7
• CCL7
• chemokine (C-C motif) ligand 7
• FIC
• MARC
• MCP-3
• MCP-3/CCL7
• MCP3Monocyte chemotactic protein 3
• MCP-3Small-inducible cytokine A7
• MGC138465
• Monocyte chemoattractant protein 3
• NC28SCYA6
• SCYA7MGC138463
• small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

Murine CCL7, also known as MARC, is belonging to the CC chemokine family. It is encoded by the gene CCL7 and was isolated from a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Similar to the human system, MARC/FIC is postulated the murine MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic fuction for monocytes and eosinophils, but not for neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL7
• Uniprot:
  • Q03366()
  • Q03366()
  • Q03366()