Recombinant Human CCL7/MCP-3/MARC Protein

SDS-Page: Human CCL7/MCP-3/MARC Protein [NBP2-34972]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CCL7/MCP-3/MARC Protein Summary

• Description: A single, non-glycosylated polypeptide chain containing 76 amino acids corresponding to CCL7/MCP-3/MARC Source: E. coli

  Uniprot ID: P80098

  Amino Acid Sequence: QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL

• Details of Functionality: MCP3 protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL7
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL7/MCP-3/MARC as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human CCL7/MCP-3/MARC Protein

• C-C motif chemokine 7
• CCL7
• chemokine (C-C motif) ligand 7
• FIC
• MARC
• MCP-3
• MCP-3/CCL7
• MCP3Monocyte chemotactic protein 3
• MCP-3Small-inducible cytokine A7
• MGC138465
• Monocyte chemoattractant protein 3
• NC28SCYA6
• SCYA7MGC138463
• small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL7
• Uniprot:
  • P80098()
  • P80098()
  • P80098()