CCL6/C10 Antibody (262016)

Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemo­taxis elicited by Recombinant Mouse CCL6/C10 (1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB487). The ND50 is typically 6‑24 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N, CyTOF-ready, Flow
• Clone: 262016
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL6/C10 Antibody (262016) Summary

• Immunogen: E. coli-derived recombinant mouse CCL6/C10
  Gly22-Ala116
  Accession # P27784
• Specificity: Detects mouse CCL6/C10 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25, 28, recombinant mouse CCL3, 4, 9, 11, 12, 19, 20, 21, 22, 24, 25, 28, or recombinant rat CCL20 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ccl6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 6-24 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL6/C10 Antibody (262016)

• C10
• c10;C-C motif chemokine 6;MRP;MRP-1;Scy;Scya6
• CCL6
• MRP-1
• SCYA6

Background

CCL6, also known as C10 and SCYA6, is a beta chemokine with an extended N-terminus. CCL6 expression is induced by GM-CSF stimulation of monocytes and neutrophils and IL-4 stimulation of macrophages. CCL6 functions as a monocyte and macrophage chemoattractant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL6/C10 Antibody (MAB487)(4)

Publications using MAB487

• W Dai, M Yang, P Xia, C Xiao, S Huang, Z Zhang, X Cheng, W Li, J Jin, J Zhang, B Wu, Y Zhang, PH Wu, Y Lin, W Wu, H Zhao, Y Zhang, WJ Lin, X Ye A functional role of meningeal lymphatics in sex difference of stress susceptibility in mice Nature Communications, 2022-08-16;13(1):4825. 2022-08-16 [PMID: 35974004] (In Vivo, Mouse)
• Michela Masetti, Roberta Carriero, Federica Portale, Giulia Marelli, Nicolò Morina, Marta Pandini et al. Lipid-loaded tumor-associated macrophages sustain tumor growth and invasiveness in prostate cancer Journal of Experimental Medicine 2022-02-07 [PMID: 34919143]
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)
• Kotarsky K, Sitnik KM, Stenstad H, Kotarsky H, Schmidtchen A, Koslowski M, Wehkamp J, Agace WW A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa. Mucosal Immunol, 2009-10-07;3(1):40-8. 2009-10-07 [PMID: 19812544] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: Ccl6
• Uniprot:
  • P27784()