CCL6/C10 Antibody (262008) [Unconjugated]

Recombinant Mouse CCL6/C10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4871) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4872) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Mu
• Applications: ELISA
• Clone: 262008
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL6/C10 Antibody (262008) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL6/C10
  Gly22-Ala116
  Accession # P27784
• Specificity: Detects mouse CCL6/C10 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL6/C10 Antibody (262008) [Unconjugated]

• C10
• c10;C-C motif chemokine 6;MRP;MRP-1;Scy;Scya6
• CCL6
• MRP-1
• SCYA6

Background

Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P27784()