CCL28 Antibody [Unconjugated]

Recombinant Human CCL28 (717-VC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human CCL28 is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF717). Typically, 100 µg/mL will cause >50% neutralization in the presence of 2 µg/mL Recombinant Human CCL28.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL28 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL28
  Ile23-Tyr127
  Accession # Q9NRJ3
• Specificity: Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CCL28 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL28
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 100 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL28 Antibody [Unconjugated]

• CCL28
• chemokine (C-C motif) ligand 28
• MEC
• member 28
• MGC71902
• Protein CCK1
• VIC

Background

Human CCL28 (CC chemokine ligand 28) is a novel CC chemokine identified by TBLASTN searches of the Human Genome Systems (HGS) and Genbank dbEst database using a human chemokine consensus sequence. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression was found to be highest in normal and pathologic colon with the protein being expressed by epithelial cells. Human CCL28 RNA was also present in normal and asthmatic lung tissues. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL28 Antibody (AF717)(4)

Publications using AF717

• Ishan Roy, Kathleen A. Boyle, Emily P. Vonderhaar, Noah P. Zimmerman, Egal Gorse, A. Craig Mackinnon et al. Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma Laboratory Investigation 3/1/2017 [PMID: 28092365]
• Ishan Roy, Kathleen A. Boyle, Emily P. Vonderhaar, Noah P. Zimmerman, Egal Gorse, A. Craig Mackinnon et al. Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma Laboratory Investigation 2017-03-01 [PMID: 28092365] (Neutralization, Human)
• Danilova , E, Skrindo , I, Gran , E, Hales , B J, Smith , W A, Jahnsen , J, Johansen , F E, Jahnsen , F L, Baekkevold , E S A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa. Mucosal Immunol, 2014-06-11;8(1):107-14. 2014-06-11 [PMID: 24917456] (IHC, Human)
• Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. J. Immunol., 2003-02-01;170(3):1452-61. 2003-02-01 [PMID: 12538707] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CCL28
• Uniprot:
  • Q9NRJ3()